Due to very high humidity inside the culture vessel and artificial conditions of development, the plantlets are tender and are therefore are not ready for coping up with the filed conditions. The plants removed from the sterile medium are washed and are maintained under intermittent mist or are covered with clean transparent plastic. After 10 to 15 days under high humidity, the plants are transferred to green house and maintained for another 4 to 6 weeks. They are then ready to be transferred to net house or the field. Normally, the tissue culture plants are sold either as ex-agar plants or hardened plants from the green house.
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